A young knight in the Order of the Sword tasked with demon-slaying. He has a notoriously twisted personality and a dirty mouth, both of which tend to drive people away and result him in being assigned the Order's "dirtiest" jobs. But Nero actually prefers solo missions, because they suit his style. His prodigious skill has earned him the respect of Captain Credo and others. His right arm has transformed into that of a demon.

The legendary devil hunter who resealed the Demon Emperor Mundus after the latter's revival. Dante is a rare human-demon hybrid, born to a human woman and the demon Sparda, who is worshipped by the Order of the Sword as a god. Dante's skills in battle with his blade and handguns are said to surpass even his father's.
Upon sensing impropriety behind the Order's actions, he attacks during the Festival of the Blade and assassinates Sanctus. Regardless of whether or not Dante is aware of Nero's lineage, he decides to watch over the young demon slayer all the same.

Vergil is Dante's twin brother, and he also carries the bloodline of Sparda. Unlike Dante, he embraces his demonic heritage. Intrigued by the Order of the Sword, who worship Sparda as a god, he pays a visit to Fortuna many years before Dante's arrival.

Vergil

A young woman who has treated Nero, an orphan, like family since they were both children. Her devout and kind nature has earned her the trust of those around her. She's also a skilled singer and, as such, was selected to be the songstress for the Festival of the Blade. Her exact relationship with Nero is unclear, at once being his family, friend, and even lover.

Kyrie's older brother and captain of the Order of the Sword. He is no empty title, however, his skills with a sword are unmatched. Credo is an exemplary leader who commands several hundred subordinates, and his strict yet fair nature has won him their undying respect. Like Kyrie, he sees Nero as family, but at the same time, Credo is endlessly incensed by Nero's rebellious tendencies.

The Order of the Sword's chief technology researcher. Agnus is responsible for developing technology and weapons with which to fight demons, and the mechanized swords used by the Order's knights are his creations. He's overzealous in his research and rarely appears at the Order's public functions. Few in the Order are even aware of Agnus' existence. He starts to stutter when excited.

An officer in the Order of the Sword and the organization's only woman. She charms those around her with her exotic looks and flirtatious mannerisms. Many in the Order are suspicious of her sudden rise to power so shortly after entering the faith. Gloria is actually Trish in disguise, she has infiltrated the organization for purposes unknown.

A demon who resembles Dante's mother. She was originally created by the Demon Emperor to lure Dante to his doom, but she had a change of heart after Dante rescued her and has been his friend ever since. Trish is known to act on a whim and go her own way sometimes, but now she's back in action alongside Dante.

A human who hunts demons for a living. She met Dante long ago, opposing him at first but eventually befriending him after many trials and tribulations. She sometimes pays Dante a visit to request help with jobs she can't quite handle on her own or to collect some of the massive amount of money he owes her.

The Order of the Sword's high priest. As a leader, Sanctus enjoys he peoples' absolute trust and is regarded as the Order's greatest high priest to date. He served as a captain of the Order's knights in his younger days. Sanctus' wealth of scholarly knowledge is unparalleled.